Cell Culture and Purification
At CuriRx, our cell culture and purification facility produces biologics that alleviate the bottlenecks or capacity shortfalls of your product development.
CuriRx welcomes projects from vector construction to harvest. Our scientists can develop an optimized batch-fed process to produce therapeutic candidates, or they can work with you to optimize your existing process. We offer industry-leading platforms and decades of experience, enhancing your team’s productivity, and we provide nonGMP expressions to produce biologics using any of the following:
- Baculovirus (Insect) Expression
- Mammalian Expression
- Bacterial Expression
- Yeast Expression
We can perform cell cultures in shake flasks, wave bags, 1L, and 10L stirred-tank bioreactors. Our team will work closely with you to customize a program for your specific application.
- Production in 1L to 10L bioreactor
- Purification steps to purify >99% or as required
- Buffer exchange
A high-yield, scalable, and cost-efficient purification process is a key component of any development program. Our scientists have created reliable and reproducible and robust processes for biologics. We employ a variety of chromatographic methods to develop entire purification trains, from harvest to polishing, using several sources, including tissues/organs, cell paste, and conditioned media. CuriRx believes in developing the most efficient and scalable processes using industry standards and approved techniques and resins.
- Viral inactivation and clearance steps will be developed to ensure that the process delivers safe and acceptable material for further processing into drug product.
- Once a process is created, we can perform robustness studies to define acceptable process parameters. Further, characterizing and profiling impurities can lead to a deeper understanding of the process and serve as a benchmark during scale-up activities.
Some of our most common chromatographic methods:
- Affinity chromatography
- Ion-exchange chromatography – cation exchange, anion exchange
- Gel filtration / size-exclusion chromatography
- Hydrophobic interaction chromatography