High Resolution Mass Spectrometry
We utilize state-of-art, high resolution mass spectrometry to fulfill all your proteomic analysis needs.
We offer the following mass spectrometric testing services. Please contact us to discuss the optimum analysis approach and with any questions on sample requirements.
• Peptide mapping
• Protein identification
• Antibody (Ab) and protein characterization
• Characterize protein conjugation (e.g. ADC)
• Intact protein analysis (intact mass analysis)
• Accurate mass measurement of peptides and proteins
• Identify and localize protein post translational modifications
• Disulfide bond mapping
• Protein/peptide quantitation
• Purity and heterogeneity of proteins and antibodies
• Protein aggregation, polymerization, and higher order structural (HOS) characterization
• Glycan and glycosylation analysis
• Protein and Antibody stability analysis
• HCP (host cell protein) analysis
• Small molecule analysis
Discovery and Targeted Proteomics
- Discovery proteomics: Confidently identify hundreds to thousands of proteins in complex samples. Global profiling of your samples allows comparison of changes in protein expression (relative quantitation) across the entire proteome. This discovery mode often proceeds more in-depth characterization of key proteins using targeted proteomic approaches.
- Targeted proteomics: Characterized and quantify (absolute quantitation) the proteins of interest in your samples. Confidently identify and quantitate a wide range of post-translational modifications. Mechanism of Action (MOA) studies detail the pharmacological effect of drug dosing by quantifying changes in key proteins in a given pathway.
Quality
We apply state-of-the-art mass spectrometry (MS) techniques and instrumentation while following USP 1055 and 621 and ICH Q6B guidelines.
Proteomics
Peptide mapping
We utilize an optimized range of digestion enzymes to maximize coverage of your protein using high resolution mass spectrometry. This can confirm a protein sequence, identify unknown proteins, and detail any sequence variants. This is often first optimization step when localizing sites of post translational modification.
An optimized digestion of your proteins is performed and searched against protein databases. Identify unknown proteins in your liquid sample and gel bands.
An optimized digestion of your proteins is performed and searched against protein databases. Identify unknown proteins in your liquid sample and gel bands.
An optimized digestion of your proteins is performed and searched against protein databases. Identify unknown proteins in your liquid sample and gel bands.
- Accurate molecular weight of reduced and/or non-reduced proteins/antibodies
- Peptide mapping (add link to peptide mapping section above)
- Localize post translational modifications including deamidation, oxidation, fragmentation, and glycosylation (in special cases methylation, N-acetylation, S nitrosylation) Ubiquitination, and phosphorylation
- Assess antibody critical quality attributes (CQA)
- Characterize structural attributes including of N- and C-termini (e.g. pyroglutamic acid, and lysine at the C-terminus of the heavy chain, etc.), free sulfhydryl and disulfide bridge structure, and glycosylation.
- Disulfide bonding mapping and characterize any disulfide scrambling
- Degradation products induced by pH, temperature, light photostability (link to photostabililty.html section), oxidation, agitation, freeze-thaw, etc. including aggregates and charge variants in samples
- Epitope mapping of antibodies
- Characterize antibody-drug conjugation (ADC) and drug-to-antibody ratio (DAR) with either covalent or non-covalent payloads
• Characterize proteins using top-down and middle down proteomics
We analyze antibodies at the protein level to characterize antibody-drug conjugation (ADC) and drug-to-antibody ratio (DAR) with either covalent or non-covalent payloads.
We analyze antibodies at the protein level to characterize antibody-drug conjugation (ADC) and drug-to-antibody ratio (DAR) with either covalent or non-covalent payloads.
We analyze antibodies at the protein level to characterize antibody-drug conjugation (ADC) and drug-to-antibody ratio (DAR) with either covalent or non-covalent payloads.